Protocol for using an ELISA to detect total α-synuclein levels in Drosophila melanogaster lines expressing human α-synuclein point mutations.
Protocol for using an ELISA to detect total α-synuclein levels in Drosophila melanogaster lines expressing human α-synuclein point mutations.
Where did the research take place?
The study site has not been established. Author addresses may differ from where the research occurred.
San Antonio, US · Author affiliation
Department of Cellular and Integrative Physiology, University of Texas San Antonio Health Science Center, Joe R. and Teresa Lozano Long School of Medicine, San Antonio, TX, USA. Electronic address: sciortino@uthscsa.edu.Location evidence
Perry, US · Author affiliation
Department of Cellular and Integrative Physiology, University of Texas San Antonio Health Science Center, Joe R. and Teresa Lozano Long School of Medicine, San Antonio, TX, USA; Center for Biomedical Neuroscience, University of Texas San Antonio Health Science Center, Joe R. and Teresa Lozano Long School of Medicine, San Antonio, TX, USA; Perry and Ruby Stevens Parkinson's Disease Center of Excellence, University of Texas San Antonio Health Science Center, Joe R. and Teresa Lozano Long School of Medicine, San Antonio, TX, USA. Electronic address: banerjees@uthscsa.edu.Location evidence
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Original abstract
In Parkinson's disease (PD), alpha-synuclein (α-syn) aggregation causes neuronal dysfunction and death, particularly of dopaminergic neurons, which is central to PD symptoms and progression. Here, we present a protocol for using a sandwich ELISA to quantify total α-syn levels in various Drosophila melanogaster genotypes expressing human α-syn point mutations associated with PD. We describe steps for aging flies, collecting fly heads, and preparing samples. We then detail procedures for ELISA setup and microplate reader analysis.