A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescence.
A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescence.
Where did the research take place?
The study site has not been established. Author addresses may differ from where the research occurred.
Montréal, CA · Author affiliation
Neuroscience and Neurosurgery, Montreal Neurological Institute-Hospital, Montreal, Québec, Canada.Location evidence
Québec, CA · Author affiliation
Neuroscience and Neurosurgery, Montreal Neurological Institute-Hospital, Montreal, Québec, Canada.Location evidence
Publication status: preprint
A plain-language reading has not been prepared for this paper yet.
Original abstract
The human GBA1 gene encodes glucocerebrosidase (GCase), a lysosomal enzyme that hydrolyzes glucosylceramides. Variants in GBA1 and reduced GCase activity have been linked to Parkinson's disease and Gaucher's disease. Here we have characterized twenty-four GCase commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.