The extent of alpha-synuclein aggregate accumulation in neurons varies depending on the type of neurotransmitter they release.
The extent of alpha-synuclein aggregate accumulation in neurons varies depending on the type of neurotransmitter they release.
Where did the research take place?
The study site has not been established. Author addresses may differ from where the research occurred.
Tokyo, JP · Author affiliation
Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan.Location evidence
Bunkyo, JP · Author affiliation
Department of Neurology, Juntendo University School of Medicine, Bunkyo- ku, Tokyo, 113-8421, Japan.Location evidence
Saitama, JP · Author affiliation
Neurodegenerative Disorders Collaboration Laboratory, RIKEN Center for Brain Science, Saitama, 351-0198, Japan.Location evidence
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Original abstract
Alpha-synuclein (aSyn) is a highly conserved 140-amino acid presynaptic protein involved in neuronal plasticity and recognized as the pathogenic protein in Parkinson's disease. Aggregated aSyn propagates transsynaptically between neurons in vivo, impairing the cellular function of affected cells. However, it remains unclear whether aggregated aSyn propagates similarly across all neuronal cell types. In this study, we injected sonicated aSyn preformed fibrils into the striatum of mice and used immunohistochemistry to observe the intracellular localization of aSyn aggregates at designated survival times. Four weeks post-injection, numerous filamentous aggregates were detected in the striatum, neocortex, and substantia nigra. Multiple immunostaining techniques with neuronal markers, combined with electron microscopy, revealed that some filamentous aggregates accumulated in the perinuclear cytoplasm of neurons in these regions. However, quantitative analyses revealed that the number of cell bodies containing aSyn aggregates in the striatum was significantly lower than that in the neocortex or substantia nigra. Additionally, we examined aSyn aggregates in the glial cells of the striatum and found that filamentous aggregates were rarely detected, even 16 weeks post-injection. These findings suggest a cell-type preference depending on neurotransmitter identity for aSyn aggregation and propagation in vivo.